INHS PaCE Lab at Turtle Survival Alliance Symposium

Three members of the INHS PaCE Lab, Dr. Michael Dreslik, Emily Asche, and Claire Dietrich, attended the 23rd Annual Symposium on the Conservation and Biology of Tortoises and Freshwater Turtles, held in Chattanooga, TN July 20-25, 2025.


Emily Asche presented a paper “Assessing Population Structure and Conservation Priorities for the Western Pond Turtle (Actinemys spp.) on California Military Installations.” The paper was co-authored with Matthew Parry, Michael Dreslik, Robert Lovich, and Thomas Akre.

Abstract: The Western Pond Turtle (Actinemys spp.) is currently under review for listing as a Threatened species under the Endangered Species Act. Widespread population declines driven by habitat loss, predation, and shell disease have led to its designation as a Species of Special Concern in California, sensitive/critical in Oregon, and endangered in Washington. Despite regional protection, federal listing remains uncertain, emphasizing the urgent need for data to guide conservation. To address critical knowledge gaps, we conducted a two-year demographic study across 13 military installations spanning the species’ California range. Standardized week-long trapping sessions yielding data on body size, life stage, and sex. The metrics were used to estimate local abundance to establish a baseline to inform population health and trajectory. Our analysis revealed variations in demographic composition, site-specific abundance, and growth dynamics across installations, identifying high-risk populations and areas with potential recovery. Our findings are essential for prioritizing conservation actions, informing management of military lands, and contributing to range-wide assessments necessary for the species’ recovery planning.


Claire Dietrich presented a poster “Initial Survey of Freshwater Turtle Assemblages at Thirteen Nature Preserves in Northeastern Illinois.” The poster was co-authored with Michael Dreslik.

Abstract: Urbanization of the Chicago metropolitan area has drastically altered the landscape of northeastern Illinois over the past two centuries. Once dominated by forest and marshy wetlands, the region is now characterized by dense urban centers highly fragmented by several major roadways. The resulting loss of natural habitat has impacted regional abundances of some turtle species, such as the state-endangered Blanding’s Turtle (Emydoidea blandingii). Despite modern environmental protections, fragmentation and degradation of the remaining habitat provide little opportunity for populations to expand or recolonize their former range naturally. We sampled thirteen publicly owned forest preserves, conservation areas, and mitigation sites for aquatic turtles with baited hoop traps to determine species richness, diversity, and evenness of the turtle assemblages. Species richness ranged from 1 to 5, with most assemblages comprising common species considered resilient to anthropogenic disturbance such as the Painted Turtle (Chrysemys picta) and Common Snapping Turtle (Chelydra serpentina). Diversity indices were low overall, ranging from 0 to 1.19, but evenness indices varied from 0.38 to 1. We intend to continually reassess turtle assemblages at the sites on a five-year rotation to gain a long-term temporal understanding of demographic trends.


Dr. Dreslik presented a paper “Population Ecology of Spotted Turtles (Clemmys guttata) in Two Isolated Illinois Wetlands,” co-authored with Devin Edmonds, Rose Arnold, and Ethan Kessler.

Abstract: Over sixty percent of evaluated freshwater turtle species are assessed as at risk of extinction by the IUCN Red List. Overexploitation is the greatest threat, with habitat degradation, urbanization, pollution, and the ongoing effects of climate change also having large impacts. Consequently, many freshwater turtle populations are small, isolated, and declining, as is the case for the Spotted turtle (Clemmys guttata). The species has declined across its range in the eastern U.S., with only two populations remaining in Illinois at the western edge of its distribution. We conducted long-term capture-mark-recapture surveys and leveraged data collected over three decades to create demographic models of growth, survival, and reproductive output formulated into an integral projection model framework. The framework evaluates the probability of population persistence and how changes in vital rates affect population growth. Our results show the benefits of integrating size-based data sources for population assessments and the value of long-term monitoring, with implications for improving conservation efforts for Spotted Turtles in Illinois and throughout their range.


 

PaCE Lab at 2025 Joint Meeting of Ichthyologists and Herpetologists

three people in front of a banner
Chitra Basyal, Rose Arnold, and Joey Cannizzaro at JMIH 2025

It’s herpetology conference season. This past week the PaCE Lab was well represented at the 2025 Joint Meeting of Ichthyologists and Herpetologists, held in St Paul Minnesota July 9-13.

Graduate students Rose Arnold, Chitra Basyal, and Joey Cannizzaro captivated audiences with their papers and oral presentations.


woman presenting scientific poster
Rose Arnold presenting at JMIH 2025

Rose Arnold presented a poster “Gauging Population Health in Urbanized Landscapes: A Body Condition Comparison of Illinois’ Remaining Spotted Turtle (Clemmys guttata) Populations.” Abstract: Urbanization has altered the hydrology and vegetation structure of many remaining wetlands in the United States. Hydrological and vegetation shifts can alter food availability and disrupt food chains, thus impairing energy flow among trophic levels. For wetland species to persist in altered habitats, managers should promptly develop strategies prioritizing the most vulnerable populations. In some cases, land managers must evaluate population health using short-term data, which can be difficult with long-lived turtle species due to extreme longevity and delayed sexual maturity. Body condition indices (BCI) are commonly used as proxy measurements of body fat, which are assumed to reflect foraging success and fitness of individuals. Body condition can change rapidly in response to resource availability, disease, and stress, making it a viable surrogate for assessing short-term population health. A population with significantly lower body conditions may indicate chronic stressors or fewer site resources. The two extant Spotted Turtle (Clemmys guttata) populations in Illinois inhabit sites within a heavily urbanized landscape and previous studies revealed differences in demography and microhabitat composition. Here, we compare body conditions between the two remaining populations of Spotted Turtles in Illinois as an additional measure of population health.


Chitra Basyal presenting at JMIH 2025

Chitra Basyal presented a talk “Structure of freshwater turtle assemblages in a highly developed ecosystem.” Abstract: Freshwater turtles play essential ecological roles in wetlands as omnivores, scavengers, food sources, and nutrient recyclers. Studying freshwater turtle assemblages is crucial because they are sentinel species when monitoring biodiversity and ecosystem health. Northern Illinois has undergone significant habitat modifications due to urbanization, agricultural expansion, and wetland loss, negatively impacting turtle populations. However, most studies in northern Illinois have primarily examined specific species rather than entire turtle assemblages. Our study aims to address the existing gap by investigating freshwater turtle assemblages across five urbanized sites in Illinois. Monitoring the sites used capture-mark-recapture surveys over an extended period; thus, we can assess species diversity, evenness, relative abundance, and richness across sites and years. Our research will determine if there are spatial and temporal variations in assemblage structure, highlight vulnerable species, and provide valuable insights into wetland health in northern Illinois.


person explaining poster to another person
Joey Cannizzaro presenting at JMIH 2025

Joseph Cannizzaro presented a poster “Effects of Vegetation Management on Predation Rates in the Eastern Massasauga Rattlesnake (Sistrurus catenatus). Abstract: Managing habitat to maximize survivorship and population growth is integral to recovering endangered species. Nearly all native tallgrass prairie habitats in the midwestern United States have been anthropogenically converted to agriculture. The decline in remaining habitat due to encroaching woody vegetation and invasive plants impacts many prairie obligate species, including the Eastern Massasauga (Sistrurus catenatus). Eastern Massasaugas requires open habitats and early successional vegetative assemblages to thermoregulate, forage, and reproduce. To reverse declines, many wildlife managers employ vegetative control practices, such as mechanical mowing and prescribed burns, to maintain early seral stages. However, conflicting evidence suggests reducing vegetative cover could lead to artificially higher or lower predation rates. We investigated the effect of early successional vegetation management on the predation rates of Eastern Massasaugas by conducting a clay model predation study. We placed models into two treatments, habitat recently managed for vegetative succession or adjacent unmanaged habitat, and scored them for predation marks. Additionally, clay models were made in two different body postures, outstretched and coiled, to investigate how body posture potentially influences snake predation rates. Our study presents the impact of recent vegetation management on predation rates and provides insights to inform future conservation efforts.

New technique aids researchers in finding elusive mussel

A new paper in Ecology by Freshwater Mollusk Ecology and Conservation Program lead Sarah Douglass and colleagues describes the use of emerging technology paired with traditional methods to try to locate a species not seen alive in Illinois in over 100 years.

The Salamander mussel Simpsonaias ambiguahas been proposed for federal listing under the US Endangered Species Act.  Despite intense surveys, live individuals were last documented in Illinois in 1906.

The Salamander Mussel differs from other mussel species in that instead of a fish species, it uses the Mudpuppy Necturus maculosus as host for its glochidia.

The emergence of environmental DNA (eDNA) techniques provides a new method to guides surveys for potential populations.

Sarah Douglass and colleagues collected samples at 8 sites in the Sangamon River to test for both Mudpuppy and Salamander Mussel eDNA. Mudpuppy eDNA was detected in samples from 4 sites, 1 of which also contained Salamander Mussel eDNA. A group of surveyors returned to that site and conducted targeted searches of preferred habitat, yielding 12 live Salamander Mussels, the first seen in Illinois in over 100 years.

Read the complete paper here

Douglass, Sarah A., Savanna Palmer, Ashleigh R. McCallum, Olivia P. Reves, Hayley A. Robinson, Allison J. Rutledge, Jordan H. Hartman, Eric R. Larson, and Mark A. Davis. 2025. Environmental DNA Reveals the Salamander Mussel Simpsonaias Ambigua Alive in Illinois, USA , after a Century in Obscurity. Ecology 106 (7): e70145. https://doi.org/10.1002/ecy.70145.

New paper on 140 years of Blanding’s Turtle data

Blanding's Turtle
Adult Blanding’s Turtle photo by R Arnold

A new paper integrated Blanding’s Turtle research data collected in Illinois over the past 140 years.  With over 60,000 encounters of approximately 7,000 individual turtles, the researchers were able to analyze causes of mortality, nesting locations, and survival.

Predators represented the greatest cause of mortality and traumatic injury overall. Human caused mortality and traumatic injury was dominated by vehicle strikes (43), followed by machinery (21), and railways (20).

57% of nests were in human modified habitats including railroads and agricultural habitats.

Current protected areas in Illinois are small and crossed by roads and railways, putting Blanding’s Turtles at risk for encountering dangerous situations. Land acquisition and/or barrier/passage systems may help mitigate mortality and improve survival prospects.

Read the full paper “Archival Data Reveals Human Impacts on Blanding’s Turtle Population Persistence”  in Journal for Nature Conservation

Three new species of Mantellid frog from Madagascar

Three new species of frogs from Madagascar were described in a paper co-authored by PACE Lab PhD candidate Devin Edmonds, led by researchers at Zoological Institute at Technische Universitat Braunschweig.

The group of frogs, Genus Guibemantis, subgenus Pandanusicola, spend their lives in the Pandanus (screw-pine) trees, which are common in the Andasibe area of Madagascar. They live and reproduce in water that pools in the leaves of the trees. While surveying the trees, the researches observed frogs that did not look like any known species Genetic testing revealed 4 new species for the region, 3 of which were new to science.

frog
Guibemantis rianasoa – Beautiful Waterfall Frog – holotype male
Guibemantis vakoa holotype male
Guibemantis ambakoana paratype female

Read an article in the Miami Herald

Read the full paper at

Hugh Gabriel, Laila-Denise Rothe, Jörn Köhler, Sandratra Rakotomanga, Devin Edmonds, Pedro Galán, Frank Glaw, Richard M. Lehtinen, Andolalao Rakotoarison and Miguel Vences. 2024. Unexpected Diversity and Co-occurrence of phytotelmic Frogs (Guibemantis) around Andasibe, one of the most intensively surveyed Amphibian Hotspots of Madagascar, and Descriptions of Three New Species.  Zootaxa. 5397(4); 451-485. DOI: 10.11646/zootaxa.5397.4.1

New publication on Timber Rattlesnake movement

PACeLab PhD Candidate Andrew Jesper co-authored a new paper with his undergrad research advisor Scott Eckert at Principia College. Jesper and Eckert radio-tracked 29 individual Crotalus horridus (13 female, 16 male) in Jersey County, Illinois.

On average, males move greater daily distances and occupy larger home ranges than females, particularly during the summer when Timber Rattlesnakes find mates. Females dispersed shorter distances from their hibernacula than males. Several snakes were tracked over multiple years, and returned to their same general range each summer. This site fidelity may limit the success of translocating adult individuals.

 

 

Abstract

Understanding the home range of imperiled reptiles is important to the design of conservation and recovery efforts. Despite numerous home range studies for the Threatened timber rattlesnake (Crotalus horridus), many have limited sample sizes or outdated analytical methods and only a single study has been undertaken in the central midwestern United States. We report on the home range size, site fidelity, and movements of C. horridus in west-central Illinois. Using VHF telemetry, we located 29 C. horridus (13 female, 16 male) over a 5-year period for a total of 51 annual records of the species’ locations and movements. We calculated annual home ranges for each snake per year using 99%, 95%, and 50% isopleths derived from Brownian Bridge utilization distributions (BBMM), and we also report 100% minimum convex polygons to be consistent with older studies. We examined the effects of sex, mass, SVL, and year on home range sizes and reported on movement metrics as well as home range fidelity using both Utilization Distribution Overlap Index (UDOI) and Bhattacharyya’s affinity (BA) statistics. The home range sizes for male and non-gravid C. horridus were 88.72 Ha (CI 63.41–110.03) and 28.06 Ha (CI 17.17–38.96) for 99% BBMM; 55.65 Ha (CI 39.36–71.93) and 17.98 (CI 10.69–25.28) for 95% BBMM; 7.36 Ha (CI 5.08–9.64) and 2.06 Ha (CI 1.26–2.87) for 50% BBMM; and 78.54Ha (CI 47.78–109.30) and 27.96 Ha (CI 7.41–48.51) for MCP. The estimated daily distance traveled was significantly greater for males (mean = 57.25 m/day, CI 49.06–65.43) than females (mean = 27.55 m/day, CI 18.99–36.12), particularly during the summer mating season. Similarly, maximum displacement distances (i.e., maximum straight-line distance) from hibernacula were significantly greater for males (mean = 2.03 km, CI 1.57–2.48) than females (mean = 1.29 km, CI 0.85–1.73], and on average, males were located further from their hibernacula throughout the entirety of their active season. We calculated fidelity to high-use areas using 11 snakes that were tracked over multiple years. The mean BBMM overlap using Bhattacharyya’s affinity (BA) for all snakes at the 99%, 95%, and 50% isopleths was 0.48 (CI 0.40–0.57), 0.40 (0.32–0.49), and 0.07 (0.05–0.10), respectively. The mean BBMM overlap for all snakes using the Utilization Distribution Overlap Index (UDOI) at the 99%, 95%, and 50% isopleths was 0.64 (CI 0.49–0.77), 0.32 (CI 0.21–0.47), and 0.02 (CI 0.01–0.05)), respectively. Our results are largely consistent with those of other studies in terms of the influence of sex on home range size and movements. The species also exhibits strong site fidelity with snakes generally using the same areas each summer, though there is far less overlap in specific (e.g., 50% UDOI) high-use areas, suggesting some plasticity in hunting areas. Particularly interesting was the tendency for snakes to disperse from specific hibernacula in the same general direction to the same general areas. We propose some possible reasons for this dispersal pattern.

Read the full article: Eckert, S.A., Jesper, A.C. Home range, site fidelity, and movements of timber rattlesnakes (Crotalus horridus) in west-central Illinois. Anim Biotelemetry 12, 1 (2024). https://doi.org/10.1186/s40317-023-00357-8

 

New paper analyzing long term data on Ornate Box Turtle survival

box turtle
Ornate Box Turtle Photo by D. Edmonds

PaCE Lab doctoral candidate Devin Edmonds has a new paper in the journal Wildlife Biology: “Evaluating Population Persistence of Ornate Box Turtles (Terrapene ornata) at the Northeast Edge of their Distribution.”

This work analyzed 34 years of data from one site and 8 years of data from another to estimate female and juvenile survival and population growth over time. Being able to look at a population over a long period of time provides a better understanding of the population. This study also highlighted the need to protect adult female Ornate Box Turtles if the populations are to persist.

This work was co-authored with members of the Wildlife Epidemiology Lab.

 

Abstract: Turtles and tortoises are among the most threatened vertebrate groups. Their life history is characterized by delayed sexual maturity and a long lifespan, making populations susceptible to decline following perturbations. Despite the urgent conservation need, we are missing estimates of basic demographic traits for many species and populations. The ornate box turtle (Terrapene ornata) is a species lacking crucial demographic data. Many populations are isolated in fragmented habitats, especially in the eastern portion of their range. We carried out long-term capture-mark-recapture surveys on two isolated populations in northern Illinois to estimate population vital rates and project population persistence with deterministic stage-based matrix models. Using 34 years of data, we estimated adult female survival = 0.974 (95% CI: 0.946–0.988) and juvenile survival = 0.867 (95% CI: 0.688–0.951) at our most intensively surveyed site. At a second site using 8 years of data, we estimated adult female survival = 0.897 (95% CI: 0.783–0.954) and juvenile survival = 0.844 (95% CI: 0.551–0.960). Despite seemingly high annual survival rates, populations declined under population projections using mean vital rates. Population growth was most sensitive to adult survival, with increasing sensitivity under more pessimistic scenarios. Our results highlight the importance of long-term demographic studies for threatened species and demonstrate protecting adult female ornate box turtles is critical for ensuring populations persist at the northern edge of their distribution.

Read the full paper:

UBAP lead and ornithologist Anastasia Rahlin presents on railway ecology

UBAP program lead and ornithologist Anastasia Rahlin was invited to present at the 2023 Railroad Environmental Conference (RREC). Her talk, titled “Railway Ecology: Using Railway Corridors to Restore Native Habitats and Preserve Biodiversity,” discussed the emerging field and made recommendations for future research.

There are 48,000 hectares of railway right of way in Illinois, 774,000 hectares in the US, and 4,122,000 hectares globally which could be managed for wildlife conservation.

Rahlin discussed where research efforts should be focused”

Collisions and wildlife crossings

    • Continue fencing high-speed railways
    • Create wildlife passages for low-vagility species

Habitat Restoration

    • Maintain native plant diversity in verges
    • Focus on restoring grasslands – appear more susceptible to invasive species

Species monitoring and management

    • Expand focus from large mammals
    • Standardized, repeated surveys needed to establish baselines
    • Need for robust occupancy and abundance models
    • Multi-year monitoring before and after construction/expansions
    • Investigate life history parameters for well-studied species
    • Adaptive management framework

Future Research

    • Before-After-Control-Impact (BACI) designs
    • Establish experimental vegetation plots along verges
    • Take advantage of novel technology

INHS PaCE Lab members present at The Wildlife Society meeting

Two members of the INHS PaCE Lab presented at The Wildlife Society annual conference November 5-9 in Louisville, KY.

scientific poster about point of care device to monitor birdsUBAP program leader and ornithologist Anastasia Rahlin presented a poster “Using Point of Care devices to assess Marsh and Sedge Wren food limitation”

Abstract:
Food limitation affects wildlife health and survival, may stem from differences in body condition or habitat quality between sites, and may be exacerbated by extreme weather events. Blood metabolites have previously been used as a food limitation index in birds. To assess changes in blood metabolites in marsh and sedge wrens, we used Point of Care devices to measure blood glucose, ketones, and triglyceride levels as short and long-term food limitation indices. We collected blood samples from wrens in May-August 2022 and 2023 in two Illinois DNR state parks and one dedicated conservation area in the Chicagoland Wilderness region over the duration of the breeding season. Our data indicate short-term food limitation may increase as the breeding season progresses for both Marsh and Sedge Wrens. Ongoing modeling will test whether body condition (age, fat and muscle scores), habitat quality (wetland extent and composition), or extreme weather (drought or flooding) best predicts glucose, ketone, and triglyceride levels over the course of the breeding season. Our findings will provide insights into physiological responses of sedge and marsh wrens to food limitations, and highlight the utility of using POC devices to rapidly measure blood metabolites in the field with minimal impacts to study species. An additional goal of this research is to use blood metabolite data to identify high-quality sites for migratory birds; our data will be used to evaluate the effectiveness of conservation areas at providing high-quality habitat. This work will inform wetland and wet meadow management strategies for migratory birds.


scientific poster about pond breeding amphibian egg massesARC program leader Ethan Kessler presented a poster “Reproductive output of forested ephemeral wetland-dependent amphibians across a modified landscape”

 

Abstract:
Forested ephemeral wetlands (FEW) support diverse communities of habitat specialist species across the eastern United States, including wetland-breeding amphibians (WBA). Due to their reliance upon FEW for breeding habitat, the location of FEW on the landscape influences population dynamics and distribution of WBA. Generally, FEW are difficult to detect due to their small size and position under the canopy, however, recent technological advances provide the ability to remotely detect FEW with great accuracy. Improved FEW detection methods enable a better understanding of how FEW characteristics and distribution influence WBA presence and abundance. We counted egg masses of two widespread WBA species, Spotted Salamanders and Wood Frogs, at 231 FEW on public lands in southern Illinois using a double observer methodology from 2020–2023. We then used local and landscape characteristics to predict egg mass abundance for each species. We found egg mass counts were highly correlated between observers for each species, but the presence and abundance of egg masses were not highly correlated between the two species. For Spotted Salamanders, we found a positive effect of wetland size on egg mass abundance but found no effect of canopy cover within a 200 m buffer. Conversely, for Wood Frogs we found no effect of wetland size, but egg mass abundance was positively associated with canopy cover within 200 m of FEW. Results from this study will provide a foundation for the estimation of WBA across broad geographic scales using discrete maps of FEW.