New technique aids researchers in finding elusive mussel

A new paper in Ecology by Freshwater Mollusk Ecology and Conservation Program lead Sarah Douglass and colleagues describes the use of emerging technology paired with traditional methods to try to locate a species not seen alive in Illinois in over 100 years.

The Salamander mussel Simpsonaias ambiguahas been proposed for federal listing under the US Endangered Species Act.  Despite intense surveys, live individuals were last documented in Illinois in 1906.

The Salamander Mussel differs from other mussel species in that instead of a fish species, it uses the Mudpuppy Necturus maculosus as host for its glochidia.

The emergence of environmental DNA (eDNA) techniques provides a new method to guides surveys for potential populations.

Sarah Douglass and colleagues collected samples at 8 sites in the Sangamon River to test for both Mudpuppy and Salamander Mussel eDNA. Mudpuppy eDNA was detected in samples from 4 sites, 1 of which also contained Salamander Mussel eDNA. A group of surveyors returned to that site and conducted targeted searches of preferred habitat, yielding 12 live Salamander Mussels, the first seen in Illinois in over 100 years.

Read the complete paper here

Douglass, Sarah A., Savanna Palmer, Ashleigh R. McCallum, Olivia P. Reves, Hayley A. Robinson, Allison J. Rutledge, Jordan H. Hartman, Eric R. Larson, and Mark A. Davis. 2025. Environmental DNA Reveals the Salamander Mussel Simpsonaias Ambigua Alive in Illinois, USA , after a Century in Obscurity. Ecology 106 (7): e70145. https://doi.org/10.1002/ecy.70145.

New publication from aquatic ecologist Hugo Ruellen

INHS PaCE Lab’s aquatic ecologist, Hugo Ruellen is lead author on a recent publication stemming from his M.Sc. research on freshwater mussels: “Predicting suitable habitat for surrogate species of critically imperiled freshwater mussels to aid in translocations.”

Mussel translocations have been used to mitigate the impacts of in-stream construction projects, including bridge replacements. This work developed a model to predict suitable habitat for mussels. “Knowing where and how much suitable habitat is present in rivers is essential to the conservation and long-term persistence of endangered freshwater mussel species, and habitat suitability models such as these are important tools for achieving these goals.”

 

Ruellan, Hugo Y., Kirk W. Stodola, Alison P. Stodola, and Jeremy S. Tiemann. 2023. Predicting suitable habitat for surrogate species of critically imperiled freshwater mussels to aid in translocations. Freshwater Science 42(3): 296-314. https://doi.org/10.1086/726740

We’re Hiring an Aquatic Ecologist

The Illinois Natural History Survey Population and Community Ecology Lab is seeking an Aquatic Ecologist to conduct basic and applied research assisting with ongoing surveys for aquatic amphibians and reptiles, freshwater mollusks, and fishes. This position will be based in Champaign, Illinois. See more or apply

 

 

Five year, $6 million contract awarded to UBAP team

The Urban Biotic Assessment Program was awarded $6,226,400 to continue providing technical assistance to the Illinois Tollway Environmental team.

This is the third intergovernmental agreement between the INHS and the Illinois Tollway and will allow the continuation of this mutually beneficial partnership that began in 2005.

The partnership has grown from monitoring Blanding’s Turtles in the Des Plaines River Valley to a multi-disciplinary program. While the key function is to provide the ecological knowledge necessary to comply with state and federal regulations, both organizations share a goal of studying the natural resources of the region to protect them into the future.

Research projects in this new agreement include biotic surveys of each of the Tollway corridors, continuation of longterm monitoring of the Kishwaukee River mussel population, using eDNA to detect cryptic species, Rusty Patched Bumble Bee surveys, and identifying bat roost trees.

Download our report of activities from 2015-2020

PACE Lab researchers awarded two Competitive State Wildlife Grants

Two projects from the INHS PACE Lab were among the 21 projects funded nationally by the 2021 U.S. Fish and Wildlife Service’s Competitive State Wildlife Grant (C-SWG) program.

The first cooperative project is with the Iowa and Illinois Department of Natural Resources, allocated at $499,797 for the joint project titled “Blanding’s Turtle Conservation in Iowa and Illinois, 2022 through 2024”.  The project team from the Illinois Natural History Survey (INHS) and National Great Rivers Research and Education Center (NGREEC) comprises Ethan J. Kessler, Michael J.  Dreslik, Andrew R. Kuhns, and John A. Crawford. The team was awarded $249,449 for their portion of the project, “Population Assessment and Space Use in a Kankakee Sands Region Blanding’s Turtle Population.” While much work has been done on Blanding’s Turtle populations in the Chicagoland region, this project focuses on lesser-known populations in the Kankakee Sands Conservation Opportunity Area.

The grant will facilitate an intensive capture-mark-recapture study to determine population size and begin to collect demographic data. A subset of turtles will be tracked using radio telemetry and GPS trackers to monitor survival and determine space and habitat use. The data is necessary to determine the amount and extent of suitable habitat available on the landscape and address causes of mortality for this population. The work will build on previous research on Blanding’s Turtles in Illinois and further inform conservation planning.

musselsThe second cooperative project is with the Minnesota Department of Natural Resources allocated at $247,892 for the joint project titled, “Regional Assessment of Widespread Mussel Declines: a Multistep Approach to Examine Potential Causes.” The project team from the INHS comprises Sarah A. Douglass and Alison P. Stodola while INHS alum Bernard Sietman will lead Minnesota’s portion. The INHS team was awarded $113,364 for their portion of the project. Freshwater mussels, an ecologically important component of river ecosystems, are experiencing widespread declines. With the award, Douglass and Stodola will investigate potential causes for these declines with the goal of informing conservation guidelines and recovery planning. Additionally, this study is an expansion of a largescale, cooperative project spearheaded by Dr. Wendell Haag, US Forest Service Research Fisheries Biologist, and American Rivers to examine causes of freshwater mussel declines across North America.

Researchers will assess pairs of rivers with similar historic mussel assemblages, comparing a river with relatively intact mussel assemblages and a river with a degraded assemblage. They will develop health metrics and assess habitat characteristics to identify potential causal factors of decline. Juvenile mussels will be propagated and used to assess health in response to potential causes of decline. Another part of the project will use eDNA and targeted sampling to update knowledge of Salamander Mussel populations in Illinois and Minnesota. The research will build on a pilot project previously undertaken by Douglass to use eDNA to detect the critically imperiled species and its host, the Mudpuppy salamander.

In granting these awards, USFWS recognizes the necessity of these two projects to further the goals of the Illinois Wildlife Action Plan to conserve imperiled species and their habitats.

 

PaCE Lab at the Illinois State Fair

Smokey Bear using snake tongs

Members of the PaCE Lab exhibited in Conservation World at the 2019 Illinois State Fair, providing information and education to over 500 visitors. In addition to displays about the research being done by the group, visitors were able to try their hand at using actual field equipment used by scientists in their daily work.

The Illinois Bat Conservation Program had a mist net deployed where visitors could untangle, identify, and measure bats, all while wearing leather gloves.

The Amphibian and Reptile Conservation group had snake tongs, hooks, calipers, and radio telemetry equipment available for visitors to try to wrangle snakes into a snake bag, measure turtles, or track a hidden turtle.

Other activities included Build-a-Bug, where people can assemble the arthropod of their dreams (or nightmares) from a variety of general and specialized appendages, Wheel of Migration, about the risks migratory birds face, and locating PIT-tagged animals.

Smokey Bear using snake tongs
Gray Treefrog picked the right tent to visit
Wheel of Migration
Build a Bug
Vin Vasive wrangling a snake with a hook
Build-a-Bug

9 days, 3 conferences, 8 talks, 2 posters

It’s been a busy week of sharing science for members of the PACE lab.

The Chicago Wilderness Wildlife Committee Meeting was held at Lincoln Park Zoo on February 19th:

 

Tara Hohoff presented “The status of Illinois bats five years after confirmation of white-nose syndrome,” using data from her work with the Illinois Bat Conservation Program and the Urban Biotic Assessment Program monitoring for the Illinois Tollway.

 

Joshua Sherwood presented “Assessing the distribution and habitat of Iowa Darters (Etheostoma exile) in Illinois,” with co-authors Andrew Stites, Jeremy Tiemann, and Michael Dreslik. This work changed the way people look for the Iowa Darter.

 

Jason Robinson presented “Patterns of abundance and co-occurrence of bumblebees associated with the Rusty Patched bumblebee.” RPBB is a federally protected species found in northeastern Illinois that has experienced a decline in its range.

 

Jason Ross presented “Demographic influence of head-starting on a Blanding’s Turtle (Emydoidea blandingii) population in DuPage County, Illinois,” with co-author Michael Dreslik, discussing what amount of head-starting is needed to keep this population viable

 

The  2019 Wild Things Conference was held in Rosemont on February 23rd:

Tara Hohoff, representing the Illinois Bat Conservation Program, presented a poster “Year Three of the Illinois Bat Conservation Program.”

Anastasia Rahlin co-presented “Secretive Marsh Birds in the Big City.” with Audubon collaborator Stephanie Beilke on their ongoing work using playback to detect 17 focal wetland bird species in northeast Illinois and southeast Indiana. Soras were the most commonly detected species which was surprising/unexpected since Marsh Wrens and Swamp Sparrows are expected to be more common, and Little Blue Herons and Yellow-headed Blackbirds were the least detected which was pretty expected due to their declines. Future directions include creating species-specific occupancy models to better understand how our focal species respond to urbanization and presence of different wetland types at three different spatial scales.

Josh Sherwood presented “Current status of Bigeye Chub (Hybopsis amblops) in Illinois”.

Sarah Douglass presented “A preliminary analysis of mussel population dynamics in the Kishwaukee River.”

Jeremy Tiemann presented “Pulling the plug – Results of the fish and mussel salvage following the removal of the Danville Dam on the Vermilion River.”

Andy Stites presented a poster “Fecundity estimates of the Gravel Chub Erimystax x-punctatus

Snakes, fish, turtles, birds, and clams – PACE Lab at Midwest Fish and Wildlife conference

The PACE Lab was well represented at this week’s Midwest  Fish and Wildlife conference in Cleveland Ohio.  Lab members and affiliates from the INHS Herp Lab and the INHS Mollusc Lab presented 7 talks, 1 lightning talk, and 1 poster on a variety of fauna including: Massasaugas, Banded Killifish, Asian Clams, Alligator Snapping Turtles, Bigeye Chub, and Rails.

Presentations

  • Tracking recovery goals for the conservation reliant Eastern Massasauga rattlesnake. M. Redmer, M.J. Dreslik, and E.T. Hileman

  • Monitoring Eastern Massasauga populations within the Carlyle Lake region. M.J. Dreslik, J.A. Crawford, S.J. Baker, and C.A. Phillips

 

 

  • Combating threats to the Eastern Massasauga with directed conservation actions in Illinois. C.A. Phillips, S.J. Baker, and M.J. Dreslik
  • The epidemiology of Snake Fungal Disease in Eastern Massasaugas over the last 10 years. M.C. Allender, E. Haynes, M. Kelley, and S.J. Baker

 

  • Rapid expansion of Banded Killifish (Fundulus diaphanous) across northern Illinois: dramatic recovery or invasive species? J.S. Tiemann, P.W. Willink, T.A. Widloe, V.J. Santucci Jr., D. Makauskas, S D. Hertel, J. T. Lamer, and J.L. Sherwood

 

  • Testing the role of stream flow eDNA abundance using the invasive Asian clam Corbicula spp. M.A. Davis, J.S. Tiemann, S.A. Douglass, and E.R. Larson

Ethan Kessler presenting

  • Can we use environmental DNA to detect Alligator Snapping Turtles (Macrochelys temminckii) at the edge of their range? E J. Kessler, K.T. Ash, S.N. Barratt, E.R. Larson, and M.A. Davis

 

 

Lightning Talks

  • Using environmental DNA to determine Rail occupancy and track migration. A.A. Rahlin, M.A. Davis, and M.L. Niemiller

Posters

  • Recovery of Bigeye Chub (Hybopsis amblops) populations in Illinois. J.L. Sherwood, A J. Stites, J.S. Tiemann, and M.J. Dreslik

The Enigmatic Asian Clam

Photo by L. Brian Stauffer Illinois News Bureau

UBAP Malacologists Sarah Douglass and Jeremy Tiemann wrote an article for the Fall 2018 issue of Illinois Audubon: “The Enigmatic Asian Clam.” Asian Clams are an invasive species that became established in the Midwest in the 1960s. Douglass and Tiemann identified an unknown species of Asian Clam found in the Illinois River in 2015 and have been studying its distribution. They plan to examine the effect of Asian Clams on the growth of native mussels in Illinois streams.

UBAP Malacologists Sarah Douglass and Jeremy Tiemann wrote an article for the Fall 2018 issue of Illinois Audubon: “The Enigmatic Asian Clam.” Asian Clams are an invasive species that became established in the Midwest in the 1960s. Douglass and Tiemann identified an unknown species of Asian Clam found in the Illinois River in 2015 and have been studying its distribution. They plan to examine the effect of Asian Clams on the growth of native mussels in Illinois streams.

The article is available from Illinois Audubon or by contacting the authors.

Illinois Audubon Magazine